Prokaryotic vs Eukaryotic

8 MCQs9-step worked example
Source: NCERT Plant KingdomPYQ coverage: NEET 2020, 2021, 2022, 2023, 2024, 2025Official key: NTA-verifiedLast reviewed: May 2026

Lesson

The high-frequency trap in prokaryotic vs eukaryotic cell comparisons: ribosome sedimentation values. Prokaryotes have 70S ribosomes (50S + 30S subunits). Eukaryotic cytoplasmic ribosomes are 80S (60S + 40S subunits). The catch — mitochondria and chloroplasts also contain 70S ribosomes due to their endosymbiotic origin. A NEET question asking "which organelle has 70S ribosomes?" expects you to pick mitochondria/chloroplast, not confuse them with the cytoplasmic 80S.

Core structural differences (NCERT Class 11 Biology Chapter 8, page 156):

  • Membrane-bound nucleus: Absent in prokaryotes (nucleoid region with naked circular DNA). Present in eukaryotes (linear DNA with histones, enclosed by nuclear envelope).
  • Membrane-bound organelles: Prokaryotes lack ER, Golgi, mitochondria, lysosomes. Eukaryotes possess all.
  • Cell size: Prokaryotes typically 1–5 μm; eukaryotes typically 10–100 μm.
  • Cell wall composition: Prokaryotes — peptidoglycan (bacteria) or pseudopeptidoglycan (archaea). Plant eukaryotes — cellulose. Animal eukaryotes — no cell wall.
  • DNA organization: Prokaryotes — single circular chromosome, no histones, plasmids common. Eukaryotes — multiple linear chromosomes wrapped around histones.
  • Reproduction: Prokaryotes — binary fission. Eukaryotes — mitosis/meiosis.

NEET watch-out: When a question lists features and asks you to identify which belongs to prokaryotes vs eukaryotes, the ribosome coefficient (70S vs 80S) is the most commonly tested discriminator. Remember that "S" values are not additive — 50S + 30S = 70S, not 80S — because Svedberg units measure sedimentation rate, not mass.

Practice MCQs

Select an option to see the explanation. Wrong answers show why your choice was tempting — and name the exact trap it exploits.

MCQ 1Easy RecallPractice

Which of the following is a characteristic feature of prokaryotic cells?

MCQ 2Easy RecallPractice

The ribosomes found in mitochondria of eukaryotic cells are:

MCQ 3Easy RecallPractice

Svedberg units (S) are not additive because they measure:

MCQ 4Easy RecallPractice

Which of the following structures is common to both prokaryotic and eukaryotic cells?

MCQ 5Direct ApplicationPractice

A student observes a cell with the following features: peptidoglycan cell wall, 70S ribosomes, circular DNA, and no membrane-bound organelles. The cell is most likely:

MCQ 6Direct ApplicationPractice

Which statement correctly distinguishes prokaryotic DNA from eukaryotic DNA?

MCQ 7Direct ApplicationPractice

An antibiotic that specifically targets 70S ribosomes would be effective against bacteria but would also potentially damage which eukaryotic organelle?

MCQ 8CalculationPractice

Consider the following cellular features:

Worked Example

Pattern: Comparison-based recall with distractor confusion (from NEET pattern: unit bundle — "similar-term-confusion" distractor type)

  1. 1

    Given

    Four ribosome sources: bacterial cytoplasm, eukaryotic cytoplasm, chloroplast, mitochondria.

  2. 2

    Required

    Count of sources containing 70S ribosomes.

  3. 3

    Concept

    Prokaryotic cells and semi-autonomous eukaryotic organelles (mitochondria, chloroplasts) have 70S ribosomes. Eukaryotic cytoplasmic ribosomes are 80S.

  4. 4

    Formula

    No formula required — this is a recall + classification question.

  5. 5

    Substitution

    - (i) E. coli (prokaryote) → 70S ✓ - (ii) Human liver cytoplasm (eukaryote) → 80S ✗ - (iii) Spinach chloroplast (endosymbiotic organelle) → 70S ✓ - (iv) Human mitochondria (endosymbiotic organelle) → 70S ✓

  6. 6

    Calculation

    Count of 70S sources = 3 (E. coli + chloroplast + mitochondria).

  7. 7

    Final answer

    **Three** of the four sources yield 70S ribosomes.

  8. 8

    Common trap

    The trap is including human liver cytoplasm as 70S (confusing the cell type with the organelle). Remember: the cytoplasmic ribosomes of ANY eukaryote are always 80S. The 70S ribosomes inside mitochondria and chloroplasts are a separate population from the cytoplasmic ones.

  9. 9

    Similar NEET-style question

    "Which of the following contains 70S ribosomes? (a) Cytoplasm of Amoeba (b) Chloroplast of Chlamydomonas (c) Cytoplasm of human RBC (d) Smooth ER of hepatocyte" Answer: (b) — chloroplast ribosomes are 70S. ---

Before solving, remember these

Key Fact

Prokaryotic vs eukaryotic

Prokaryotes: no membrane-bound nucleus, smaller (1-10 μm), 70S ribosomes, no true organelles, single circular DNA, peptidoglycan cell wall (bacteria). Eukaryotes: membrane-bound nucleus, 80S ribosomes (70S in mito/chloroplast), multiple linear chromosomes, organelles.

-- NCERT Class 11 Biology, Ch. 8, p. 156

Formulas

Michaelis-Menten equation

Initial reaction velocity v of an enzyme as a function of substrate concentration. Km = [S] at v = Vmax/2.

SymbolQuantitySI Unit
vrate-
Vmaxmax rate-
KmMichaelis constantM
[S]substrateM

Valid when

  • Single substrate
  • No allosteric/cooperative effects

Exam Traps & Common Mistakes

These are the exact patterns that cause wrong answers in NEET. Each trap includes when it triggers and how to avoid it.

Trap

Mitosis vs meiosis stage characteristics swapped

Category: Similar Terms

Synapsis, crossing over, chiasmata are meiosis-only (prophase I). Equational mitosis has none.

When it triggers

Question describes a phase event and asks which division it belongs to.

How to avoid

Synapsis/CO/chiasmata = meiosis I. Sister chromatid separation = mitosis or meiosis II. Homologue separation = meiosis I.

Trap

70S vs 80S ribosome confusion

Category: Similar Terms

Prokaryotes 70S; eukaryotic cytosol 80S; mitochondria + chloroplast 70S (endosymbiotic).

When it triggers

Question gives organelle/cell type and asks ribosome type.

How to avoid

Cytosolic of eukaryote = 80S; everywhere else (bacteria, mito, chloroplast) = 70S.

Past Year Questions

83 questions from NEET 2020, 2021, 2022, 2023, 2024, 2025. Answers verified against NTA official keys.

NEET 2025

Given below are two statements : One is labelled as Assertion (A) and other is labelled as Reason (R). Assertion (A) : Cells of the tapetum possess dense cytoplasm and generally have more than one nucleus. Reason (R) : Presence of more than one nucleus in the tapetum increases the efficiency of nourishing the developing microspore mother cells. In light of the above statements, choose the most appropriate answer from the options given below:

1A is false but R is true
2Both A and R are true and R is the correct explanation of A
3Both A and R are true but R is NOT the correct explanation of A
4A is true but R is false
NTA Answer: Option 4(final)
NEET 2025

Histones are enriched with -

1Phenylalanine & Arginine
2Lysine & Arginine
3Leucine & Lysine
4Phenylalanine & Leucine
NTA Answer: Option 2(final)
NEET 2025

Given below are two statements : one is labelled as Assertion (A), and the other is labelled as Reason (R). Assertion (A) : The primary function of the Golgi apparatus is to package the materials made by the endoplasmic reticulum and deliver it to intracellular targets and outside the cell. Reason (R) : Vesicles containing materials made by the endoplasmic reticulum fuse with the cis face of the Golgi apparatus, and they are modified and released from the trans face of the Golgi apparatus. In the light of the above statements, choose the correct answer from the options given below :

1A is false but R is true
2Both A and R are true and R is the correct explanation of A
3Both A and R are true but R is not the correct explanation of A
4A is true but R is false
NTA Answer: Option 3(final)
NEET 2025

From the statements given below choose the correct option : A. The eukaryotic ribosomes are 80S and prokaryotic ribosomes are 70S. B. Each ribosome has two sub-units. C. The two sub-units of 80S ribosome are 60S and 40S while that of 70S are 50S and 30S. D. The two sub-units of 80S ribosome are 60S and 20S and that of 70S are 50S and 20S. E. The two sub-units of 80S are 60S and 30S and that of 70S are 50S and 30S.

1B, D, E are true
2A, B, C are true
3A, B, D are true
4A, B, E are true
NTA Answer: Option 2(final)
NEET 2025

Given below are two statements : Statement I : Transfer RNAs and ribosomal RNA do not interact with mRNA. Statement II : RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence. In the light of the above statements, choose the most appropriate answer from the options given below :

1Statement I is incorrect but statement II is correct
2Both statement I and statement II are correct
3Both statement I and statement II are incorrect
4Statement I is correct but statement II is incorrect
NTA Answer: Option 1(final)
NEET 2024

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. B. It may get integrated into the genome of the recipient. C. It may multiply and be inherited along with the host DNA. D. The alien piece of DNA is not an integral part of chromosome. E. It shows ability to replicate. Choose the correct answer from the options given below:

1A and B only
2D and E only
3B and C only
4A and E only
NTA Answer: Option 3(final)
NEET 2024

Given below are two statements: Statement I : Chromosomes become gradually visible under light microscope during leptotene stage. Statement II : The beginning of diplotene stage is recognized by dissolution of synaptonemal complex. In the light of the above statements, choose the correct answer from the options given below:

1Both Statement I and Statement II are true
2Both Statement I and Statement II are false
3Statement I is true but Statement II is false
4Statement I is false but Statement II is true
NTA Answer: Option 1(final)
NEET 2024

The DNA present in chloroplast is:

1Linear, double stranded
2Circular, double stranded
3Linear, single stranded
4Circular, single stranded
NTA Answer: Option 2(final)
NEET 2024

Read the following statements and choose the set of correct statements: In the members of Phaeophyceae, A. Asexual reproduction occurs usually by biflagellate zoospores. B. Sexual reproduction is by oogamous method only. C. Stored food is in the form of carbohydrates which is either mannitol or laminarin. D. The major pigments found are chlorophyll a, c and carotenoids and xanthophyll. E. Vegetative cells have a cellulosic wall, usually covered on the outside by gelatinous coating of algin. Choose the correct answer from the options given below:

1A, B, C and D only
2B, C, D and E only
3A, C, D and E only
4A, B, C and E only
NTA Answer: Option 3(final)
NEET 2024

Given below are two statements: Statement I: Mitochondria and chloroplasts both double membranes bound organelles. Statement II: Inner membrane of mitochondria is relatively less permeable, as compared chloroplast. In the light of the above statements, choose the mis appropriate answer from the options given below

1Both Statement I and Statement II are correct.
2Both Statement I and Statement II are incorrect.
3Statement I is correct but Statement Il is incorrect.
4Statement I is incorrect but Statement Il is correct.
NTA Answer: Option 3(final)
NEET 2023

Given below are two statements: Statement I: A protein is imagined as a line, the left end represented by first amino acid (C-terminal) and the right end represented by last amino acid (N-terminal). Statement II: Adult human haemoglobin, consists of 4 subunits (two subunits of - 35- type and two subunits of type.) In the light of the above statements, choose the correct answer from the options given below:

1Both Statement I and Statement II are false.
2Statement I is true but Statement II is false.
3Statement I is false but Statement II is true.
4Both Statement I and Statement II are true
NTA Answer: Option 3(final)
NEET 2023

Given below are two statements : Statement I : Low temperature preserves the enzyme in a temporarily inactive state whereas high temperature destroys enzymatic activity because proteins are denatured by heat. Statement II : When the inhibitor closely resembles the substrate in its molecular structure and inhibits the activity of the enzyme, it is known as competitive inhibitor. In the light of the above statements, choose the correct answer from the options given below :

1Both Statement I and Statement II are false.
2Statement I is true but Statement II is false.
3Statement I is false but Statement II is true.
4Both Statement I and Statement II are true.
NTA Answer: Option 4(final)
NEET 2023

Given below are two statements: Statement I : During G phase of cell cycle, the cell is metabolically inactive. 0 Statement II : The centrosome undergoes duplication during S phase of interphase. In the light of the above statements, choose the most appropriate answer from the options given below:

1Both Statement I and Statement II are incorrect.
2Statement I is correct but Statement II is incorrect.
3Statement I is incorrect but Statement II is correct.
4Both Statement I and Statement II are correct
NTA Answer: Option 3(final)
NEET 2022

Given below are two statements : Statement I : The primary CO acceptor in C plants is phosphoenolpyruvate and is found in the mesophyll cells. 2 4 Statement II : Mesophyll cells of C plants lack RuBisCo enzyme. In the light of the above statements, choose the correct 4 answer from the options given below:

1Statement I is incorrect but Statement II is correct
2Both Statement I and Statement II are correct
3Both Statement I and Statement II are incorrect
4Statement I is correct but Statement II is incorrect
NTA Answer: Option 2(final)
NEET 2022

Match List-I with List-II List-I List-II (a) Manganese (i) Activates the enzyme catalase (b) Magnesium (ii) Required for pollen germination (c) Boron (iii) Activates enzymes of respiration (d) Iron (iv) Functions in splitting of water during photosynthesis Choose the correct answer from the options given below :

1(a)-(iii), (b)-(i), (c)-(ii), (d)-(iv)
2(a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
3(a)-(iv), (b)-(iii), (c)-(ii), (d)-(i)
4(a)-(iv), (b)-(i), (c)-(ii), (d)-(iii)
NTA Answer: Option 3(final)
NEET 2022

Read the following statements on lipids and find out correct set of statements: (a) Lecithin found in the plasma membrane is a glycolipid (b) Saturated fatty acids possess one or more c = c bonds (c) Gingely oil has lower melting point, hence remains as oil in winter (d) Lipids are generally insoluble in water but soluble in some organic solvents (e) When fatty acid is esterified with glycerol, monoglycerides are formed Choose the correct answer from the option given below:

1(a), (b) and (d) only
2(a), (b) and (c) only
3(a), (d) and (e) only
4(c), (d) and (e) only
NTA Answer: Option 4(final)
NEET 2022

Match List-I with List-II. List-I List-II (a) Metacentric chromosome (i) Centromere situated close to the end forming one extremely short and one very long arms (b) Acrocentric chromosome (ii) Centromere at the terminal end (c) Submetacentric (iii) Centromere in the middle forming two equal arms of chromosomes (d) Telocentric chromosome (iv) Centromere slightly away from the middle forming one shorter arm and one longer arm Choose the correct answer from the options given below :

1(a)-(i), (b)-(ii), (c)-(iii), (d)-(iv)
2(a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
3(a)-(i), (b)-(iii), (c)-(ii), (d)-(iv)
4(a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
NTA Answer: Option 2(final)
NEET 2022

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : Mendel’s law of Independent assortment does not hold good for the genes that are located closely on the same chromosome. Reason (R) : Closely located genes assort independently. In the light of the above statements, choose the correct answer from the options given below:

1(A) is not correct but (R) is correct
2Both (A) and (R) are correct and (R) is the correct explanation of (A)
3Both (A) and (R) are correct but (R) is not the correct explanation of (A)
4(A) is correct but (R) is not correct
NTA Answer: Option 4(final)
NEET 2022

Regarding Meiosis, which of the statements is incorrect?

1Four haploid cells are formed at the end of Meiosis-II
2There are two stages in Meiosis, Meiosis-I and II
3DNA replication occurs in S phase of Meiosis-II
4Pairing of homologous chromosomes and recombination occurs in Meiosis-I
NTA Answer: Option 3(final)
NEET 2022

Which of the following statements are true for spermatogenesis but do not hold true for Oogenesis? (a) It results in the formation of haploid gametes (b) Differentiation of gamete occurs after the completion of meiosis (c) Meiosis occurs continuously in a mitotically dividing stem cell population (d) It is controlled by the Luteinising hormone (LH) and Follicle Stimulating Hormone (FSH) secreted by the anterior pituitary (e) It is initiated at puberty Choose the most appropriate answer from the options given below:

1(b), (c) and (e) only
2(c) and (e) only
3(b) and (c) only
4(b), (d) and (e) only
NTA Answer: Option 1(final)
NEET 2022

Which of the following is a correct match for disease and its symptoms?

1Muscular dystrophy – An auto immune disorder causing progressive degeneration of skeletal muscle
2Arthritis – Inflammed joints
3Tetany – High Ca2+ level causing rapid spasms.
4Myasthenia gravis – Genetic disorder resulting in weakening and paralysis of skeletal muscle
NTA Answer: Option 2(final)
NEET 2021

Identify the correct statement. (b)G 2 phase (ii) Inactive phase (c) Quiescent (iii) Interval between mitosis

1Split gene arrangement is characteristic of stage and initiation of prokaryotes DNA replication (d)G phase (iv) DNA replication 1
2In capping, methyl guanosine triphosphate is added to the 3' end of hnRNA (a) (b) (c) (d)
3RNA polymerase binds with Rho factor to (1) (ii) (iv) (iii) (i) terminate the process of transcription in bacteria (2) (iii) (ii) (i) (iv)
4The coding strand in a transcription unit is (3) (iv) (ii) (iii) (i) copied to an mRNA (4) (iv) (i) (ii) (iii)
NTA Answer: Option 3(final)
NEET 2020

∑ȧ¿U Áfl÷ÊÁ¡Ã „Ê ⁄U„Ë ∑§Ê Á‡Ê∑§Êÿ ∑§Ê Á‡Ê∑§Ê ø∑˝§áÊ ‚ ’Ê„⁄U 95. Some dividing cells exit the cell cycle and enter ÁŸ∑§‹ ¡ÊÃË „Ò •ÊÒ⁄U ∑§ÊÁÿ∑§ ÁŸÁc∑˝§ÿÃÊ •flSÕÊ ◊ ¬˝fl ‡Ê ∑§⁄U vegetative inactive stage. This is called quiescent stage (G ). This process occurs at the end of : ¡ÊÃË „Ò– ß‚ ‡ÊÊ Ã •flSÕÊ (G ) ∑§„Ê ¡ÊÃÊ „Ò– ÿ„ ¬˝Á∑˝§ÿÊ 0 0 Á∑§‚∑ § •ãà ◊ „Ê ÃË „Ò?

1G phase 2 (1) G ¬˝ÊflSÕÊ
2M phase 2 (2) M ¬˝ÊflSÕÊ
3G 1 phase
4S phase (3) G ¬˝ÊflSÕÊ 1 (4) S ¬˝ÊflSÕÊ
NTA Answer: Option 3(final)
NEET 2020

©Ÿ ¬ŒÊÕÊ Z ∑§Ê ¬„øÊÁŸ∞, Á¡Ÿ∑§Ë ‚ ⁄UøŸÊ•Ê ◊ ∑˝§◊‡Ê— 126. Identify the substances having glycosidic bond and peptide bond, respectively in their structure : Ç‹Êß∑§Ê ‚ÊßÁ«U∑§ ’ œ •ÊÒ⁄U ¬ å≈UÊß«U ’ œ ¬Êÿ ¡Êà „Ò —

1Inulin, insulin (1) ߟÈÁ‹Ÿ, ß ‚ÈÁ‹Ÿ
2Chitin, cholesterol (2) ∑§ÊßÁ≈UŸ, ∑§Ê ‹ S≈U⁄UÊÚ‹
3Glycerol, trypsin (3) ÁÇ‹‚⁄UÊÚ‹, Á≈˛UÁå‚Ÿ
4Cellulose, lecithin (4) ‚ ‹È‹Ê ¡, ‹ Á‚ÁÕŸ
NTA Answer: Option 1(final)

How NEET usually asks this

Recurring question shapes from past papers. Each pattern shows why wrong options look tempting.

Cell theory, organelles, biomolecules, enzymes, cell cycle, mitosis/meiosis stages

RecallMedium

Common distractors

enzyme subunit terminology swap

Apoenzyme, holoenzyme, cofactor, coenzyme, and prosthetic group cluster tightly. Students who have not anchored each term structurally reach for the nearest-sounding label (e.g., selecting 'coenzyme' or 'cofactor' when the answer is 'apoenzyme' for the protein part alone).

enzyme inhibition type confusion

When malonate (structural analog of succinate) inhibits succinate dehydrogenase, students with uncertain vocabulary fall for 'feedback inhibition' because they associate inhibition with a product feedback loop, even though malonate is a substrate analog competing for the active site.

meiosis stage event misassignment

The five Prophase I sub-stages have rhyming names and adjacent events. Students confuse recombination nodule appearance (Pachytene, when crossing-over occurs) with synapsis initiation (Zygotene), or place centromere division in Anaphase I instead of Anaphase II.

endomembrane membership error

Mitochondria and chloroplasts are membrane-bound organelles, so students assume they belong to the endomembrane system. The actual criterion (vesicle-mediated ER-Golgi secretory network) is not recalled under exam pressure; peroxisomes are also frequently misclassified.

metabolic pathway compartment swap

Because glycolysis feeds the Krebs cycle (which is in the mitochondrial matrix), students assume glycolysis itself also occurs in the mitochondria. The distinction cytosol=glycolysis, matrix=Krebs, inner membrane=ETS is lost under time pressure.

assertion reason unchecked reason

A/R questions with a true Assertion invite accepting the Reason without independent verification. A plausible-sounding R alongside a true A leads to selecting 'both true, R explains A' (option 2) when R is factually incorrect or logically irrelevant to A — a direct negative-mark risk.

organelle substructure recall error

Match-list questions on organelle sub-structures (axoneme/cilia, cartwheel/centriole, crista/mitochondria, satellite/chromosome) test fine-grained recall. Students who hold only a coarse concept of each organelle confuse structurally related components that share the same cell region.

mitosis meiosis phase conflation

Mitosis and meiosis share named phases but have fundamentally different events. Students conflate Anaphase I (homologous chromosomes separate, centromeres intact) with Anaphase II (centromeres split, sister chromatids separate), or attribute spindle fiber attachment to the wrong division entirely.

prokaryotic eukaryotic ribosome confusion

Students know eukaryotes use 80S ribosomes but forget that organelles of endosymbiotic origin (mitochondria, chloroplasts) retain 70S ribosomes inside eukaryotic cells. They also confuse prokaryotic (70S = 50S + 30S) with eukaryotic (80S = 60S + 40S) subunit composition.

Sources

NCERT refs: Class 11 Biology Chapter 8, p.156

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