Photoperiodism Vernalization

8 MCQs9-step worked example
Source: NCERT Animal KingdomPYQ coverage: NEET 2020, 2021, 2022, 2023, 2024, 2025Official key: NTA-verifiedLast reviewed: May 2026

Lesson

The trap that costs marks here: confusing photoperiodism with vernalization, or swapping the plant categories (short-day, long-day, day-neutral) with the wrong examples. NEET questions on this topic rely on precise recall of which plant belongs to which photoperiodic category and what vernalization actually requires.

Photoperiodism is the physiological response of plants to the relative length of day and night (photoperiod) that governs the timing of flowering. The phenomenon was first described by Garner and Allison (1920). The critical factor is actually the duration of the dark period (scotophase), not the light period — a frequent source of confusion.

Plants are classified by their photoperiodic response:

  • Short-day plants (SDP): Flower when the dark period exceeds a critical length. Examples: rice, tobacco, chrysanthemum, soybean, Xanthium.
  • Long-day plants (LDP): Flower when the dark period is shorter than a critical length. Examples: wheat, oat, spinach, radish, sugar beet.
  • Day-neutral plants (DNP): Flowering is independent of photoperiod. Examples: tomato, cucumber, cotton, sunflower.

The photoreceptor involved is phytochrome — specifically the interconversion between Pr (red-absorbing, inactive) and Pfr (far-red-absorbing, active). A flash of red light during a long dark period can prevent flowering in SDPs because it converts Pr → Pfr, effectively "breaking" the dark period.

Vernalization is the promotion of flowering by exposure to low temperature (typically 0–5°C) for a defined period. Winter wheat and biennial plants like carrots and cabbage require vernalization. The hormone vernalin was proposed (though not conclusively isolated) as the stimulus. Vernalization acts on the shoot apex.

Key distinction for NEET: photoperiodism = light-dark duration response; vernalization = cold-temperature requirement. Both influence flowering time, but through entirely different environmental cues (NCERT Class 11 Biology Chapter 15, page 293).


Practice MCQs

Select an option to see the explanation. Wrong answers show why your choice was tempting — and name the exact trap it exploits.

MCQ 1Easy RecallPractice

Photoperiodism in plants was first studied by:

MCQ 2Easy RecallPractice

Which of the following is a short-day plant?

MCQ 3Concept TrapPractice

The critical factor that determines flowering in photoperiodic plants is:

MCQ 4Direct ApplicationPractice

A flash of red light given during the dark period to a short-day plant will:

MCQ 5Easy RecallPractice

Vernalization is the process of promoting flowering by:

MCQ 6Easy RecallPractice

The site of perception of vernalization stimulus in a plant is:

MCQ 7CalculationPractice

If a long-day plant is kept under short-day conditions but the dark period is interrupted by a brief flash of red light followed immediately by a flash of far-red light, the plant will:

MCQ 8Direct ApplicationPractice

Which of the following correctly distinguishes photoperiodism from vernalization?

Worked Example

  1. 1

    Given

    A researcher grows *Xanthium* (a short-day plant) under controlled conditions. The plant is exposed to 8 hours of light and 16 hours of darkness. During the 10th hour of darkness, a 5-minute flash of red light is given.

  2. 2

    Required

    Will the plant flower? Justify using the phytochrome model.

  3. 3

    Concept

    Short-day plants require an uninterrupted dark period exceeding a critical length to flower. The photoreceptor phytochrome exists in two interconvertible forms: Pr (red-absorbing, biologically inactive for this response) and Pfr (far-red-absorbing, biologically active). Red light converts Pr → Pfr.

  4. 4

    Relevant principle

    In SDPs, continuous darkness allows Pfr (accumulated during the light period) to slowly revert to Pr. If the dark period is uninterrupted and long enough, Pfr levels drop below a threshold, permitting flowering. A red-light flash during the dark period converts Pr back to Pfr, resetting the "clock" and effectively breaking the continuous dark period.

  5. 5

    Substitution / application

    - Dark period provided: 16 hours (sufficient for *Xanthium*). - Red-light flash at hour 10: converts Pr → Pfr. - After the flash, the remaining dark period is only 6 hours — likely below the critical dark-period threshold. - Pfr levels are now elevated, which inhibits flowering in SDPs.

  6. 6

    Reasoning

    The red-light interruption at the 10th hour creates two short dark segments (10 h and 6 h) instead of one continuous 16-hour dark period. Neither segment, from the plant's perspective after phytochrome resetting, satisfies the critical uninterrupted dark-period requirement.

  7. 7

    Final answer

    The plant will **not flower**. The red-light flash broke the continuous dark period by converting Pr → Pfr, preventing the SDP flowering response.

  8. 8

    Common trap

    Confusing the effect of red vs. far-red light: if the flash had been far-red light, it would convert Pfr → Pr and would NOT inhibit SDP flowering (the dark period would remain effectively unbroken for the phytochrome response). NEET questions often test this red/far-red reversal.

  9. 9

    Similar NEET-style question

    "A short-day plant is kept under 8 h light / 16 h dark. A red-light flash is given at the 8th hour of darkness, followed immediately by a far-red flash. Will the plant flower? Explain." (Answer: Yes — far-red reverses the red effect, restoring Pr dominance, so the dark period is effectively unbroken.) ---

Before solving, remember these

Photoperiodism: response to relative duration of light/dark. Short-day plants (Xanthium — flowering when night > critical), long-day plants (Henbane), day-neutral. Phytochrome: red/far-red receptor. Vernalization: cold treatment to induce flowering (winter wheat).

-- NCERT Class 11 Biology, Ch. 15, p. 293

Exam Traps & Common Mistakes

These are the exact patterns that cause wrong answers in NEET. Each trap includes when it triggers and how to avoid it.

Category: Similar Terms

C3: 3-PGA (3-carbon) is first stable product. C4: oxaloacetate (4-carbon) is first stable product.

When it triggers

Question asks first stable product of CO2 fixation in C3/C4 plant.

How to avoid

Pathway name = carbons in first product. C3 → 3C; C4 → 4C.

Category: Negative Marking

Light reactions: water splits at PS II (P680) FIRST, electrons flow PS II → PS I (P700). PS II numbered AFTER PS I in discovery, but reaction order is PS II → PS I.

When it triggers

Question on Z-scheme order, water photolysis, electron source.

How to avoid

Discovery order ≠ reaction order. Photolysis at PS II; PS II reduces PS I.

Root cause: concept gap

Correction

Glycolysis is in CYTOPLASM (cytosol). Krebs cycle in mitochondrial matrix; ETC in inner membrane.

Past Year Questions

38 questions from NEET 2020, 2021, 2022, 2023, 2024, 2025. Answers verified against NTA official keys.

NEET 2025

Given below are two statements : Statement I : Fig fruit is a non-vegetarian fruit as it has enclosed fig wasps in it. Statement II : Fig wasp and fig tree exhibit mutual relationship as fig wasp completes its life cycle in fig fruit and fig fruit gets pollinated by fig wasp. In the light of the above statements, choose the most appropriate answer from the options given below :

1Statement I is incorrect but statement II is correct
2Both statement I and statement II are correct
3Both statement I and statement II are incorrect
4Statement I is correct but statement II is incorrect
NTA Answer: Option 3(final)
NEET 2025

Which are correct: A. Computed tomography and magnetic resonance imaging detect cancers of internal organs. B. Chemotherapeutics drugs are used to kill non-cancerous cells. C. -interferon activate the cancer patients’ immune system and helps in destroying the tumour. D. Chemotherapeutic drugs are biological response modifiers. E. In the case of leukaemia blood cell counts are decreased. Choose the correct answer from the options given below:

1A and C only
2B and D only
3D and E only
4C and D only
NTA Answer: Option 1(final)
NEET 2024

Given below are two statements: Statement I: In C plants, some O binds to RuBisCO, hence CO fixation is decreased. 3 2 2 Statement II: In C plants, mesophyll cells show very little photorespiration while bundle sheath cells do not 4 show photorespiration. In the light of the above statements, choose the correct answer from the options given below:

1Both Statement I and Statement II are true
2Both Statement I and Statement II are false
3Statement I is true but Statement II is false
4Statement I is false but Statement II is true
NTA Answer: Option 3(final)
NEET 2023

Given below are two statements : One is labelled as Assertion A and the other is labelled as Reason R : Assertion A : ATP is used at two steps in glycolysis. Reason R : First ATP is used in converting glucose into glucose-6-phosphate and second ATP is used in conversion of fructose-6-phosphate into fructose-1, 6-diphosphate. In the light of the above statements, choose the correct answer from the options given below :

1Both A and R are true but R is NOT the correct explanation of A.
2A is true but R is false.
3A is false but R is true.
4Both A and R are true and R is the correct explanation of A.
NTA Answer: Option 4(final)
NEET 2021

Which of the following statements is correct ? cambial ring

1Some of the organisms can fix atmospheric Answer (4) nitrogen in specialized cells called sheath cells 138. Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance.
2Fusion of two cells is called Karyogamy If this enzyme is used for inserting a gene for β-galactoside production and the recombinant
3Fusion of protoplasms between two motile on non-motile gametes is called plasmogamy plasmid is inserted in an E.coli strain (1) It will be able to produce a novel protein with
4Organisms that depend on living plants are dual ability called saprophytes (2) It will not be able to confer ampicillin resistance
NTA Answer: Option 3(final)
NEET 2020

•ÁŸflÊÿ ¸ÃàflÊ •Ê⁄Ò U ¬ÊŒ¬Ê ◊ ©Ÿ∑§ ∑§ÊÿÊ Z ∑§ Áfl ÿ ◊ ÁŸêŸÁ‹Áπà 102. Match the following concerning essential elements and their functions in plants : ∑§Ê ‚È◊ Á‹Ã ∑§ËÁ¡∞ — (a) Iron (i) Photolysis of water (a) ‹Ê „ (i) ¡‹ ∑§Ê ¬˝∑§Ê‡Ê •¬ÉÊ≈UŸ (b) Zinc (ii) Pollen germination (b) Á¡∑ § (ii) ¬⁄Uʪ ∑§Ê • ∑ȧ⁄UáÊ (c) Boron (iii) Required for chlorophyll (c) ’Ê⁄ UÊŸÚ (iii) Ä‹Ê ⁄UÊ Á»§‹ ∑ § ¡Òfl ‚ ‡‹ áÊ biosynthesis ∑ § Á‹∞ •Êfl‡ÿ∑§ (d) Manganese (iv) IAA biosynthesis (d) ◊Ò ªŸË¡ (iv) •Ê߸.∞.∞. ¡Òfl ‚ ‡‹ áÊ Select the correct option : ‚„Ë Áfl∑§À¬ øÈÁŸ∞ — (a) (b) (c) (d) (a) (b) (c) (d)

1(iv) (i) (ii) (iii) (1) (iv) (i) (ii) (iii)
2(ii) (i) (iv) (iii) (2) (ii) (i) (iv) (iii)
3(iv) (iii) (ii) (i) (3) (iv) (iii) (ii) (i)
4(iii) (iv) (ii) (i) (4) (iii) (iv) (ii) (i)
NTA Answer: Option 4(final)
NEET 2020

⁄UÊÁòÊ ◊ ÿÊ ¬Íáʸ ¬˝Ê×∑§Ê‹ ◊ ÉÊÊ‚ ∑§Ë ¬ÁûÊÿÊ ∑ § ‡ÊË ¸ ‚ ¡‹ ∑ § 134. The process responsible for facilitating loss of water Œ˝fl •flSÕÊ ◊ ÁŸ∑§‹Ÿ ∑§Ê ‚Ȫ◊ ’ŸÊŸ ◊ ∑§ÊÒŸ ‚Ë ¬˝Á∑˝§ÿÊ in liquid form from the tip of grass blades at night ©ûÊ⁄UŒÊÿË „Ê ÃË „Ò? and in early morning is :

1¡ËflŒ˝√ÿ∑È §øŸ (1) Plasmolysis
2flÊc¬Êà ‚¡Ÿ¸ (2) Transpiration
3◊Í‹Ëÿ ŒÊ’ (3) Root pressure
4•à —‡ÊÊ áÊ (4) Imbibition
NTA Answer: Option 3(final)
NEET 2020

ÁŸêŸÁ‹Áπà ◊ ‚ ∑§ÊÒŸ ∞∑§ ’Ë¡ ¬˝‚ÈÁåà ÁŸÿ ÁòÊà ∑§⁄UŸ flÊ‹Ê 135. Which of the following is not an inhibitory ÁŸ⁄UÊ œ∑§ ¬ŒÊÕ¸ Ÿ„Ë „Ò? substance governing seed dormancy ?

1¬Ò⁄UÊ-∞ S∑§ÊÚÁ’¸∑§ •ê‹ (1) Para-ascorbic acid
2Á¡’⁄ UÁ‹∑§ •ê‹ (2) Gibberellic acid
3∞é‚ËÁ‚∑§ •ê‹ (3) Abscisic acid
4Á»§ŸÊ Á‹∑§ •ê‹ (4) Phenolic acid Hindi+English 31 H3
NTA Answer: Option 2(final)

How NEET usually asks this

Recurring question shapes from past papers. Each pattern shows why wrong options look tempting.

Mineral nutrition, photosynthesis, respiration, growth, hormones, photoperiodism

RecallMedium

Common distractors

photosynthesis pathway product confusion

C3 and C4 pathways share the Calvin cycle; students who cannot anchor the first stable product in each pathway invert the carbon counts: 3-PGA (3-carbon) is the C3 first stable product, OAA (4-carbon) is the C4 first stable product. RuBisCO has dual carboxylase and oxygenase activity; students who recall only one role select incorrect statements about its function, including denying that it causes photorespiration.

ps i ps ii assignment reversal

PS I and PS II are numbered in order of discovery, not order of action in the Z-scheme. PS II acts first (water photolysis, O2 release, P680 reaction centre at 680 nm); PS I acts second (P700 reaction centre at 700 nm, NADPH production). Students who use Roman numeral order assign water photolysis to PS I and attribute the 700 nm absorption maximum to PS II.

respiration compartment swap

Glycolysis feeds the mitochondrial Krebs cycle, creating a mental link: students place glycolysis in the mitochondrial matrix. The anchor -- glycolysis occurs in the cytosol because it predates mitochondria and operates in anaerobes -- is lost under time pressure.

rubisco function confusion

RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) has dual activity: carboxylase drives CO2 fixation in the Calvin cycle; oxygenase drives photorespiration. Questions give multiple statements about RuBisCO and ask which set is correct; students who have not anchored the dual-function name omit or deny the oxygenase role, selecting option sets that describe it as carboxylase only.

assertion reason unchecked reason

A/R questions in plant physiology present a true Assertion (e.g., ATP is used at two steps in glycolysis) alongside a Reason that sounds mechanistically plausible but may be factually imprecise or not the correct explanation. Students accept the Reason without independently checking whether its specific biochemical claim is accurate.

Sources

NCERT refs: Class 11 Biology Chapter 15, p.293

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