Photosynthesis Chloroplast

8 MCQs9-step worked example
Source: NCERT Animal KingdomPYQ coverage: NEET 2020, 2021, 2022, 2023, 2024, 2025Official key: NTA-verifiedLast reviewed: May 2026

Lesson

The chloroplast is the organelle where photosynthesis happens — and NEET questions on this topic test whether you actually know its internal architecture or are guessing from vague diagrams.

Structure. Chloroplasts are double-membrane-bound organelles found in mesophyll cells. The outer membrane is permeable to small molecules; the inner membrane is selective. Inside lies the stroma — the aqueous matrix — and suspended within the stroma is a third membrane system: the thylakoid membrane. Thylakoids are flattened, disc-like sacs. Where thylakoids stack into columns, the stack is called a granum (plural: grana). Thylakoids connecting adjacent grana are called stroma lamellae (or intergranal thylakoids). The space enclosed within each thylakoid is the lumen (NCERT Class 11 Biology Chapter 13, page 244).

Pigments. Photosynthetic pigments are embedded in the thylakoid membrane. The primary pigment is chlorophyll a — the only pigment that directly participates in the light reactions by donating an excited electron to the reaction centre. Chlorophyll b, xanthophylls, and carotenoids are accessory pigments: they absorb wavelengths that chlorophyll a cannot and transfer that energy to chlorophyll a. Carotenoids also protect chlorophyll from photo-oxidation.

Where the confusion hits. A common NEET trap is confusing the site of the light reactions (thylakoid membrane, specifically the grana thylakoids) with the site of the Calvin cycle (stroma). Another frequent error: calling stroma lamellae the same thing as grana — they are NOT stacked, and they lack PS II.

Watch out: Questions may ask what is present in stroma lamellae versus grana thylakoids. Stroma lamellae contain PS I but lack PS II, meaning they carry out only cyclic photophosphorylation.


Practice MCQs

Select an option to see the explanation. Wrong answers show why your choice was tempting — and name the exact trap it exploits.

MCQ 1Easy RecallPractice

How many membrane layers does a chloroplast possess?

MCQ 2Easy RecallPractice

Which pigment directly participates in the photochemical reaction at the reaction centre?

MCQ 3Easy RecallPractice

The space enclosed within the thylakoid membrane is called the:

MCQ 4Direct ApplicationPractice

A student observes that stroma lamellae can perform cyclic photophosphorylation but not non-cyclic photophosphorylation. Which of the following best explains this observation?

MCQ 5Direct ApplicationPractice

A chloroplast extract is treated so that all carotenoid pigments are selectively removed. Which of the following consequences is most likely under high light intensity?

MCQ 6Direct ApplicationPractice

Which of the following correctly matches the chloroplast compartment with a process that occurs there?

MCQ 7Concept TrapPractice

A researcher isolates intact chloroplasts and separates the grana fraction from the stroma fraction. The grana fraction is illuminated but provided with no CO₂. Which products will this fraction generate?

MCQ 8CalculationPractice

Consider the following statements about chloroplast pigments:

Worked Example

  1. 1

    Given

    A question states: "In chloroplasts, the__(A)__ is the site of light reactions, while the__(B)__ is the site of the Calvin cycle. The__(C)__ connect grana stacks and contain PS I but not PS II."

  2. 2

    Required

    Identify the three chloroplast structures A, B, and C.

  3. 3

    Concept

    The chloroplast has distinct compartments: the thylakoid membrane system (further divided into grana thylakoids and stroma lamellae) and the stroma. Each compartment hosts specific reactions.

  4. 4

    Formula / Principle

    No formula applies. The principle is compartment–function mapping: light reactions → thylakoid membrane of grana; Calvin cycle → stroma; stroma lamellae → PS I only (cyclic photophosphorylation).

  5. 5

    Substitution / Application

    - (A): Light reactions occur on the thylakoid membrane, specifically the grana thylakoids where both PS I and PS II are located. - (B): The Calvin cycle enzymes (including RuBisCO) are dissolved in the stroma. - (C): Stroma lamellae (intergranal thylakoids) connect adjacent grana and contain PS I but lack PS II.

  6. 6

    Calculation

    No arithmetic required. This is a direct terminology-mapping question.

  7. 7

    Final answer

    A = grana thylakoid membrane, B = stroma, C = stroma lamellae.

  8. 8

    Common trap

    Confusing stroma lamellae with grana. NEET may present "stroma lamellae" as a distractor for "site of non-cyclic photophosphorylation." Since stroma lamellae lack PS II, they cannot perform non-cyclic electron flow — only cyclic photophosphorylation generating ATP without NADPH.

  9. 9

    Similar NEET-style question

    "Which chloroplast structure contains PS I but not PS II, and can carry out only cyclic photophosphorylation?" Options: (a) Grana thylakoid (b) Stroma lamella (c) Inner membrane (d) Outer membrane Answer: (b) Stroma lamella. ---

Before solving, remember these

Chloroplast: outer + inner membrane; thylakoids stacked into grana; stroma. Pigments: chl a (P680, P700 reaction centres), chl b, xanthophylls, carotenoids. Action spectrum: rate of photosynthesis vs wavelength; matches absorption of chl a.

-- NCERT Class 11 Biology, Ch. 13, p. 244

Exam Traps & Common Mistakes

These are the exact patterns that cause wrong answers in NEET. Each trap includes when it triggers and how to avoid it.

Category: Similar Terms

C3: 3-PGA (3-carbon) is first stable product. C4: oxaloacetate (4-carbon) is first stable product.

When it triggers

Question asks first stable product of CO2 fixation in C3/C4 plant.

How to avoid

Pathway name = carbons in first product. C3 → 3C; C4 → 4C.

Category: Negative Marking

Light reactions: water splits at PS II (P680) FIRST, electrons flow PS II → PS I (P700). PS II numbered AFTER PS I in discovery, but reaction order is PS II → PS I.

When it triggers

Question on Z-scheme order, water photolysis, electron source.

How to avoid

Discovery order ≠ reaction order. Photolysis at PS II; PS II reduces PS I.

Root cause: concept gap

Correction

Glycolysis is in CYTOPLASM (cytosol). Krebs cycle in mitochondrial matrix; ETC in inner membrane.

Past Year Questions

38 questions from NEET 2020, 2021, 2022, 2023, 2024, 2025. Answers verified against NTA official keys.

NEET 2025

Given below are two statements : Statement I : Fig fruit is a non-vegetarian fruit as it has enclosed fig wasps in it. Statement II : Fig wasp and fig tree exhibit mutual relationship as fig wasp completes its life cycle in fig fruit and fig fruit gets pollinated by fig wasp. In the light of the above statements, choose the most appropriate answer from the options given below :

1Statement I is incorrect but statement II is correct
2Both statement I and statement II are correct
3Both statement I and statement II are incorrect
4Statement I is correct but statement II is incorrect
NTA Answer: Option 3(final)
NEET 2025

Which are correct: A. Computed tomography and magnetic resonance imaging detect cancers of internal organs. B. Chemotherapeutics drugs are used to kill non-cancerous cells. C. -interferon activate the cancer patients’ immune system and helps in destroying the tumour. D. Chemotherapeutic drugs are biological response modifiers. E. In the case of leukaemia blood cell counts are decreased. Choose the correct answer from the options given below:

1A and C only
2B and D only
3D and E only
4C and D only
NTA Answer: Option 1(final)
NEET 2024

Given below are two statements: Statement I: In C plants, some O binds to RuBisCO, hence CO fixation is decreased. 3 2 2 Statement II: In C plants, mesophyll cells show very little photorespiration while bundle sheath cells do not 4 show photorespiration. In the light of the above statements, choose the correct answer from the options given below:

1Both Statement I and Statement II are true
2Both Statement I and Statement II are false
3Statement I is true but Statement II is false
4Statement I is false but Statement II is true
NTA Answer: Option 3(final)
NEET 2023

Given below are two statements : One is labelled as Assertion A and the other is labelled as Reason R : Assertion A : ATP is used at two steps in glycolysis. Reason R : First ATP is used in converting glucose into glucose-6-phosphate and second ATP is used in conversion of fructose-6-phosphate into fructose-1, 6-diphosphate. In the light of the above statements, choose the correct answer from the options given below :

1Both A and R are true but R is NOT the correct explanation of A.
2A is true but R is false.
3A is false but R is true.
4Both A and R are true and R is the correct explanation of A.
NTA Answer: Option 4(final)
NEET 2021

Which of the following statements is correct ? cambial ring

1Some of the organisms can fix atmospheric Answer (4) nitrogen in specialized cells called sheath cells 138. Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance.
2Fusion of two cells is called Karyogamy If this enzyme is used for inserting a gene for β-galactoside production and the recombinant
3Fusion of protoplasms between two motile on non-motile gametes is called plasmogamy plasmid is inserted in an E.coli strain (1) It will be able to produce a novel protein with
4Organisms that depend on living plants are dual ability called saprophytes (2) It will not be able to confer ampicillin resistance
NTA Answer: Option 3(final)
NEET 2020

•ÁŸflÊÿ ¸ÃàflÊ •Ê⁄Ò U ¬ÊŒ¬Ê ◊ ©Ÿ∑§ ∑§ÊÿÊ Z ∑§ Áfl ÿ ◊ ÁŸêŸÁ‹Áπà 102. Match the following concerning essential elements and their functions in plants : ∑§Ê ‚È◊ Á‹Ã ∑§ËÁ¡∞ — (a) Iron (i) Photolysis of water (a) ‹Ê „ (i) ¡‹ ∑§Ê ¬˝∑§Ê‡Ê •¬ÉÊ≈UŸ (b) Zinc (ii) Pollen germination (b) Á¡∑ § (ii) ¬⁄Uʪ ∑§Ê • ∑ȧ⁄UáÊ (c) Boron (iii) Required for chlorophyll (c) ’Ê⁄ UÊŸÚ (iii) Ä‹Ê ⁄UÊ Á»§‹ ∑ § ¡Òfl ‚ ‡‹ áÊ biosynthesis ∑ § Á‹∞ •Êfl‡ÿ∑§ (d) Manganese (iv) IAA biosynthesis (d) ◊Ò ªŸË¡ (iv) •Ê߸.∞.∞. ¡Òfl ‚ ‡‹ áÊ Select the correct option : ‚„Ë Áfl∑§À¬ øÈÁŸ∞ — (a) (b) (c) (d) (a) (b) (c) (d)

1(iv) (i) (ii) (iii) (1) (iv) (i) (ii) (iii)
2(ii) (i) (iv) (iii) (2) (ii) (i) (iv) (iii)
3(iv) (iii) (ii) (i) (3) (iv) (iii) (ii) (i)
4(iii) (iv) (ii) (i) (4) (iii) (iv) (ii) (i)
NTA Answer: Option 4(final)
NEET 2020

⁄UÊÁòÊ ◊ ÿÊ ¬Íáʸ ¬˝Ê×∑§Ê‹ ◊ ÉÊÊ‚ ∑§Ë ¬ÁûÊÿÊ ∑ § ‡ÊË ¸ ‚ ¡‹ ∑ § 134. The process responsible for facilitating loss of water Œ˝fl •flSÕÊ ◊ ÁŸ∑§‹Ÿ ∑§Ê ‚Ȫ◊ ’ŸÊŸ ◊ ∑§ÊÒŸ ‚Ë ¬˝Á∑˝§ÿÊ in liquid form from the tip of grass blades at night ©ûÊ⁄UŒÊÿË „Ê ÃË „Ò? and in early morning is :

1¡ËflŒ˝√ÿ∑È §øŸ (1) Plasmolysis
2flÊc¬Êà ‚¡Ÿ¸ (2) Transpiration
3◊Í‹Ëÿ ŒÊ’ (3) Root pressure
4•à —‡ÊÊ áÊ (4) Imbibition
NTA Answer: Option 3(final)
NEET 2020

ÁŸêŸÁ‹Áπà ◊ ‚ ∑§ÊÒŸ ∞∑§ ’Ë¡ ¬˝‚ÈÁåà ÁŸÿ ÁòÊà ∑§⁄UŸ flÊ‹Ê 135. Which of the following is not an inhibitory ÁŸ⁄UÊ œ∑§ ¬ŒÊÕ¸ Ÿ„Ë „Ò? substance governing seed dormancy ?

1¬Ò⁄UÊ-∞ S∑§ÊÚÁ’¸∑§ •ê‹ (1) Para-ascorbic acid
2Á¡’⁄ UÁ‹∑§ •ê‹ (2) Gibberellic acid
3∞é‚ËÁ‚∑§ •ê‹ (3) Abscisic acid
4Á»§ŸÊ Á‹∑§ •ê‹ (4) Phenolic acid Hindi+English 31 H3
NTA Answer: Option 2(final)

How NEET usually asks this

Recurring question shapes from past papers. Each pattern shows why wrong options look tempting.

Mineral nutrition, photosynthesis, respiration, growth, hormones, photoperiodism

RecallMedium

Common distractors

photosynthesis pathway product confusion

C3 and C4 pathways share the Calvin cycle; students who cannot anchor the first stable product in each pathway invert the carbon counts: 3-PGA (3-carbon) is the C3 first stable product, OAA (4-carbon) is the C4 first stable product. RuBisCO has dual carboxylase and oxygenase activity; students who recall only one role select incorrect statements about its function, including denying that it causes photorespiration.

ps i ps ii assignment reversal

PS I and PS II are numbered in order of discovery, not order of action in the Z-scheme. PS II acts first (water photolysis, O2 release, P680 reaction centre at 680 nm); PS I acts second (P700 reaction centre at 700 nm, NADPH production). Students who use Roman numeral order assign water photolysis to PS I and attribute the 700 nm absorption maximum to PS II.

respiration compartment swap

Glycolysis feeds the mitochondrial Krebs cycle, creating a mental link: students place glycolysis in the mitochondrial matrix. The anchor -- glycolysis occurs in the cytosol because it predates mitochondria and operates in anaerobes -- is lost under time pressure.

rubisco function confusion

RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) has dual activity: carboxylase drives CO2 fixation in the Calvin cycle; oxygenase drives photorespiration. Questions give multiple statements about RuBisCO and ask which set is correct; students who have not anchored the dual-function name omit or deny the oxygenase role, selecting option sets that describe it as carboxylase only.

assertion reason unchecked reason

A/R questions in plant physiology present a true Assertion (e.g., ATP is used at two steps in glycolysis) alongside a Reason that sounds mechanistically plausible but may be factually imprecise or not the correct explanation. Students accept the Reason without independently checking whether its specific biochemical claim is accurate.

Sources

NCERT refs: Class 11 Biology Chapter 13, p.244

Test yourself on this topic with real past-paper questions:

Practice this topic →

Free NEET study resources

Get a structured 30-day study plan and a complete formula booklet — delivered to your inbox instantly.