Respiration Glycolysis

8 MCQs9-step worked example
Source: NCERT Animal KingdomPYQ coverage: NEET 2020, 2021, 2022, 2023, 2024, 2025Official key: NTA-verifiedLast reviewed: May 2026

Lesson

The single most tested confusion about glycolysis is where it happens. Students who have spent weeks on mitochondrial pathways begin to assume every respiratory step occurs inside the mitochondrion. Glycolysis does not. It occurs in the cytoplasm (cytosol) of every living cell — prokaryotic or eukaryotic, aerobic or anaerobic. No membrane-bound organelle is required.

Glycolysis is a sequence of 10 enzyme-catalysed reactions that converts one molecule of glucose (6C) into two molecules of pyruvic acid (3C). The pathway is common to both aerobic and anaerobic respiration — it is the universal entry point.

Net yield per glucose molecule:

  • 2 ATP (4 produced, 2 consumed in the preparatory phase)
  • 2 NADH + H⁺

The pathway has two phases. The preparatory (energy-investment) phase (steps 1–5) phosphorylates glucose twice using 2 ATP, splitting the 6C sugar into two 3C molecules (glyceraldehyde-3-phosphate, G3P). The payoff (energy-harvest) phase (steps 6–10) oxidises G3P, generating 4 ATP by substrate-level phosphorylation and 2 NADH by reduction of NAD⁺.

Key NCERT fact: glycolysis was discovered by Gustav Embden, Otto Meyerhof, and Jakub Parnas — hence the name EMP pathway (NCERT Class 11 Biology, Chapter 14, page 266).

Watch-out for NEET: When a question says "net gain of ATP in glycolysis," the answer is 2, not 4. When it asks "total ATP produced," the answer is 4. Read the stem word — net vs total — before marking.

Glycolysis does not require oxygen. Whether the cell is aerobic or anaerobic, glycolysis proceeds identically up to pyruvate. What happens to pyruvate after glycolysis depends on the organism and oxygen availability.


Practice MCQs

Select an option to see the explanation. Wrong answers show why your choice was tempting — and name the exact trap it exploits.

MCQ 1Easy RecallPractice

Glycolysis occurs in which part of the cell?

MCQ 2Easy RecallPractice

The net gain of ATP molecules during glycolysis from one molecule of glucose is:

MCQ 3Easy RecallPractice

Glycolysis is also known as the EMP pathway. EMP stands for:

MCQ 4Direct ApplicationPractice

During glycolysis, one molecule of glucose (6C) is broken down into:

MCQ 5Direct ApplicationPractice

Which of the following statements about glycolysis is correct?

MCQ 6Direct ApplicationPractice

In the preparatory phase of glycolysis, 2 ATP molecules are consumed. These ATP molecules are used for:

MCQ 7CalculationPractice

If 10 molecules of glucose undergo complete glycolysis, the total number of NADH molecules produced and the total number of ATP molecules consumed in the preparatory phase are, respectively:

MCQ 8Concept TrapPractice

A student claims that glycolysis cannot occur in red blood cells because mature mammalian RBCs lack mitochondria. This claim is:

Worked Example

  1. 1

    Given

    A cell undergoes glycolysis of 5 glucose molecules under anaerobic conditions.

  2. 2

    Required

    Calculate: (a) total pyruvic acid molecules produced, (b) net ATP gained, (c) total NADH produced.

  3. 3

    Concept

    Glycolysis converts 1 glucose (6C) → 2 pyruvic acid (3C). Per glucose: gross ATP = 4, ATP consumed = 2, net ATP = 2, NADH produced = 2. These yields are identical under aerobic and anaerobic conditions — glycolysis itself does not use oxygen.

  4. 4

    Formula

    - Pyruvic acid = 2 × (number of glucose molecules) - Net ATP = 2 × (number of glucose molecules) - NADH = 2 × (number of glucose molecules)

  5. 5

    Substitution

    - Pyruvic acid = 2 × 5 = 10 molecules - Net ATP = 2 × 5 = 10 molecules - NADH = 2 × 5 = 10 molecules

  6. 6

    Calculation

    All multipliers here (2 and 5) are exact counting integers — they do not limit significant figures. - Pyruvic acid produced: **10 molecules** - Net ATP gained: **10 ATP** - NADH produced: **10 NADH**

  7. 7

    Final answer

    From 5 glucose molecules undergoing glycolysis: **10 pyruvic acid, 10 net ATP, 10 NADH**. The constants 2 (stoichiometric yield per glucose) and 5 (given glucose count) are exact integers and do not affect significant-figure analysis.

  8. 8

    Common trap

    The trap is confusing *net* with *total* ATP. Total ATP produced = 4 × 5 = 20, but 2 × 5 = 10 ATP are consumed in the preparatory phase. If the question says "net gain," the answer is 10, not 20. Read the stem carefully. A second common trap: placing glycolysis in the mitochondria. The question said "anaerobic conditions," which might tempt students to think the pathway relocates — it does not. Glycolysis is always in the cytoplasm.

  9. 9

    Similar NEET-style question

    "If 8 molecules of glucose undergo glycolysis, what is the net gain of ATP and the total NADH produced?" (Answer: 16 ATP net, 16 NADH) ---

Before solving, remember these

Cytoplasm; glucose (6C) → 2 pyruvate (3C). Net yield: 2 ATP, 2 NADH. Investment phase: 2 ATP used. Payoff phase: 4 ATP, 2 NADH. Rate-limiting enzyme: phosphofructokinase.

-- NCERT Class 11 Biology, Ch. 14, p. 266

Exam Traps & Common Mistakes

These are the exact patterns that cause wrong answers in NEET. Each trap includes when it triggers and how to avoid it.

Category: Similar Terms

C3: 3-PGA (3-carbon) is first stable product. C4: oxaloacetate (4-carbon) is first stable product.

When it triggers

Question asks first stable product of CO2 fixation in C3/C4 plant.

How to avoid

Pathway name = carbons in first product. C3 → 3C; C4 → 4C.

Category: Negative Marking

Light reactions: water splits at PS II (P680) FIRST, electrons flow PS II → PS I (P700). PS II numbered AFTER PS I in discovery, but reaction order is PS II → PS I.

When it triggers

Question on Z-scheme order, water photolysis, electron source.

How to avoid

Discovery order ≠ reaction order. Photolysis at PS II; PS II reduces PS I.

Root cause: concept gap

Correction

Glycolysis is in CYTOPLASM (cytosol). Krebs cycle in mitochondrial matrix; ETC in inner membrane.

Past Year Questions

38 questions from NEET 2020, 2021, 2022, 2023, 2024, 2025. Answers verified against NTA official keys.

NEET 2025

Given below are two statements : Statement I : Fig fruit is a non-vegetarian fruit as it has enclosed fig wasps in it. Statement II : Fig wasp and fig tree exhibit mutual relationship as fig wasp completes its life cycle in fig fruit and fig fruit gets pollinated by fig wasp. In the light of the above statements, choose the most appropriate answer from the options given below :

1Statement I is incorrect but statement II is correct
2Both statement I and statement II are correct
3Both statement I and statement II are incorrect
4Statement I is correct but statement II is incorrect
NTA Answer: Option 3(final)
NEET 2025

Which are correct: A. Computed tomography and magnetic resonance imaging detect cancers of internal organs. B. Chemotherapeutics drugs are used to kill non-cancerous cells. C. -interferon activate the cancer patients’ immune system and helps in destroying the tumour. D. Chemotherapeutic drugs are biological response modifiers. E. In the case of leukaemia blood cell counts are decreased. Choose the correct answer from the options given below:

1A and C only
2B and D only
3D and E only
4C and D only
NTA Answer: Option 1(final)
NEET 2024

Given below are two statements: Statement I: In C plants, some O binds to RuBisCO, hence CO fixation is decreased. 3 2 2 Statement II: In C plants, mesophyll cells show very little photorespiration while bundle sheath cells do not 4 show photorespiration. In the light of the above statements, choose the correct answer from the options given below:

1Both Statement I and Statement II are true
2Both Statement I and Statement II are false
3Statement I is true but Statement II is false
4Statement I is false but Statement II is true
NTA Answer: Option 3(final)
NEET 2023

Given below are two statements : One is labelled as Assertion A and the other is labelled as Reason R : Assertion A : ATP is used at two steps in glycolysis. Reason R : First ATP is used in converting glucose into glucose-6-phosphate and second ATP is used in conversion of fructose-6-phosphate into fructose-1, 6-diphosphate. In the light of the above statements, choose the correct answer from the options given below :

1Both A and R are true but R is NOT the correct explanation of A.
2A is true but R is false.
3A is false but R is true.
4Both A and R are true and R is the correct explanation of A.
NTA Answer: Option 4(final)
NEET 2021

Which of the following statements is correct ? cambial ring

1Some of the organisms can fix atmospheric Answer (4) nitrogen in specialized cells called sheath cells 138. Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance.
2Fusion of two cells is called Karyogamy If this enzyme is used for inserting a gene for β-galactoside production and the recombinant
3Fusion of protoplasms between two motile on non-motile gametes is called plasmogamy plasmid is inserted in an E.coli strain (1) It will be able to produce a novel protein with
4Organisms that depend on living plants are dual ability called saprophytes (2) It will not be able to confer ampicillin resistance
NTA Answer: Option 3(final)
NEET 2020

•ÁŸflÊÿ ¸ÃàflÊ •Ê⁄Ò U ¬ÊŒ¬Ê ◊ ©Ÿ∑§ ∑§ÊÿÊ Z ∑§ Áfl ÿ ◊ ÁŸêŸÁ‹Áπà 102. Match the following concerning essential elements and their functions in plants : ∑§Ê ‚È◊ Á‹Ã ∑§ËÁ¡∞ — (a) Iron (i) Photolysis of water (a) ‹Ê „ (i) ¡‹ ∑§Ê ¬˝∑§Ê‡Ê •¬ÉÊ≈UŸ (b) Zinc (ii) Pollen germination (b) Á¡∑ § (ii) ¬⁄Uʪ ∑§Ê • ∑ȧ⁄UáÊ (c) Boron (iii) Required for chlorophyll (c) ’Ê⁄ UÊŸÚ (iii) Ä‹Ê ⁄UÊ Á»§‹ ∑ § ¡Òfl ‚ ‡‹ áÊ biosynthesis ∑ § Á‹∞ •Êfl‡ÿ∑§ (d) Manganese (iv) IAA biosynthesis (d) ◊Ò ªŸË¡ (iv) •Ê߸.∞.∞. ¡Òfl ‚ ‡‹ áÊ Select the correct option : ‚„Ë Áfl∑§À¬ øÈÁŸ∞ — (a) (b) (c) (d) (a) (b) (c) (d)

1(iv) (i) (ii) (iii) (1) (iv) (i) (ii) (iii)
2(ii) (i) (iv) (iii) (2) (ii) (i) (iv) (iii)
3(iv) (iii) (ii) (i) (3) (iv) (iii) (ii) (i)
4(iii) (iv) (ii) (i) (4) (iii) (iv) (ii) (i)
NTA Answer: Option 4(final)
NEET 2020

⁄UÊÁòÊ ◊ ÿÊ ¬Íáʸ ¬˝Ê×∑§Ê‹ ◊ ÉÊÊ‚ ∑§Ë ¬ÁûÊÿÊ ∑ § ‡ÊË ¸ ‚ ¡‹ ∑ § 134. The process responsible for facilitating loss of water Œ˝fl •flSÕÊ ◊ ÁŸ∑§‹Ÿ ∑§Ê ‚Ȫ◊ ’ŸÊŸ ◊ ∑§ÊÒŸ ‚Ë ¬˝Á∑˝§ÿÊ in liquid form from the tip of grass blades at night ©ûÊ⁄UŒÊÿË „Ê ÃË „Ò? and in early morning is :

1¡ËflŒ˝√ÿ∑È §øŸ (1) Plasmolysis
2flÊc¬Êà ‚¡Ÿ¸ (2) Transpiration
3◊Í‹Ëÿ ŒÊ’ (3) Root pressure
4•à —‡ÊÊ áÊ (4) Imbibition
NTA Answer: Option 3(final)
NEET 2020

ÁŸêŸÁ‹Áπà ◊ ‚ ∑§ÊÒŸ ∞∑§ ’Ë¡ ¬˝‚ÈÁåà ÁŸÿ ÁòÊà ∑§⁄UŸ flÊ‹Ê 135. Which of the following is not an inhibitory ÁŸ⁄UÊ œ∑§ ¬ŒÊÕ¸ Ÿ„Ë „Ò? substance governing seed dormancy ?

1¬Ò⁄UÊ-∞ S∑§ÊÚÁ’¸∑§ •ê‹ (1) Para-ascorbic acid
2Á¡’⁄ UÁ‹∑§ •ê‹ (2) Gibberellic acid
3∞é‚ËÁ‚∑§ •ê‹ (3) Abscisic acid
4Á»§ŸÊ Á‹∑§ •ê‹ (4) Phenolic acid Hindi+English 31 H3
NTA Answer: Option 2(final)

How NEET usually asks this

Recurring question shapes from past papers. Each pattern shows why wrong options look tempting.

Mineral nutrition, photosynthesis, respiration, growth, hormones, photoperiodism

RecallMedium

Common distractors

photosynthesis pathway product confusion

C3 and C4 pathways share the Calvin cycle; students who cannot anchor the first stable product in each pathway invert the carbon counts: 3-PGA (3-carbon) is the C3 first stable product, OAA (4-carbon) is the C4 first stable product. RuBisCO has dual carboxylase and oxygenase activity; students who recall only one role select incorrect statements about its function, including denying that it causes photorespiration.

ps i ps ii assignment reversal

PS I and PS II are numbered in order of discovery, not order of action in the Z-scheme. PS II acts first (water photolysis, O2 release, P680 reaction centre at 680 nm); PS I acts second (P700 reaction centre at 700 nm, NADPH production). Students who use Roman numeral order assign water photolysis to PS I and attribute the 700 nm absorption maximum to PS II.

respiration compartment swap

Glycolysis feeds the mitochondrial Krebs cycle, creating a mental link: students place glycolysis in the mitochondrial matrix. The anchor -- glycolysis occurs in the cytosol because it predates mitochondria and operates in anaerobes -- is lost under time pressure.

rubisco function confusion

RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) has dual activity: carboxylase drives CO2 fixation in the Calvin cycle; oxygenase drives photorespiration. Questions give multiple statements about RuBisCO and ask which set is correct; students who have not anchored the dual-function name omit or deny the oxygenase role, selecting option sets that describe it as carboxylase only.

assertion reason unchecked reason

A/R questions in plant physiology present a true Assertion (e.g., ATP is used at two steps in glycolysis) alongside a Reason that sounds mechanistically plausible but may be factually imprecise or not the correct explanation. Students accept the Reason without independently checking whether its specific biochemical claim is accurate.

Sources

NCERT refs: Class 11 Biology Chapter 14, p.266

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